Isaac Manke's Stock Option Grant at Q32 Bio Inc.

Isaac Manke, a director at Q32 Bio Inc., was granted 53,521 stock options on October 23, 2025. The options, which are for the purchase of common stock, have an exercise price of $2.80 per share and expire on October 22, 2035. The options will vest and become exercisable in full on the earlier of October 23, 2026, or the date of the issuer's next annual meeting of stockholders, subject to Manke's continued service on the vesting date. This transaction is reported under Form 4, indicating a change in beneficial ownership.